|
| Status |
Public on Dec 04, 2008 |
| Title |
iHMC_control_6h_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
human mesangial cell, control, 6h
|
| Organism |
Homo sapiens |
| Characteristics |
human mesangial cell from a 16-weeks-old female fetus with 36 h after spontaneous abortion; primary cell obtained from Clonetics Corp. (San Diego, CA), and later immortalized with a SV40 large T antigen expression plasmid.
|
| Biomaterial provider |
Transformed cell kindly provided by Dr. Bernard Banas, from Nephrological Center, Medical Policlinic, Ludwig-Maximilians University, Munich, Germany.
|
| Treatment protocol |
cells were cultured in serum-containg media until confluence, when cells were kept for 24 hours in DMEM 0.5% FBS. Cells were stimulated with 10% FBS or serum free-medium for 6 hours.
|
| Growth protocol |
Cells were grown in low-glucose Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (serum), containing 2 g/L sodium bicarbonate and antibiotics at pH 7.4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN RNeasy Mini Kit
|
| Label |
streptavidin-Cy5
|
| Label protocol |
Two micrograms of total RNA was labeled according to manufacturer's protocol (CodeLink Expression Analysis Tutorial Version 4.0).
|
| |
|
| Hybridization protocol |
Ten micrograms of fragmented target cRNA was used for hybridization of each UniSet Human I Expression Bioarray chip as described in manufacturer's protocol (CodeLink Expression Analysis Tutorial Version 4.0).
|
| Scan protocol |
Processed slides were scanned using an Axon GenePix Scanner with the laser set to 635 nm, 600V, 100% power laser and the scan resolution to 10 µm.
|
| Description |
RNA extracted from 3 culture dishes was pooled, producing one sample. A total of three samples (9 culture dishes) were hybridized for each group (1 samples/array).
|
| Data processing |
Slides were scanned using CodeLinkTM Expression Scanning Software and images for each slide analyzed using the CodeLinkTM Expression Analysis Software and Microsoft Excel.
|
| |
|
| Submission date |
Dec 05, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Rodrigo Tambellini |
| Organization name |
Federal university of Sao Paulo
|
| Street address |
Rua Boticatu, 740
|
| City |
Sao Paulo |
| ZIP/Postal code |
04023-900 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL4191 |
| Series (1) |
| GSE9787 |
human mesangial cell serum-treated for 6 h |
|
