|
| Status |
Public on Feb 15, 2010 |
| Title |
3624T early stage CRC patient's tumor |
| Sample type |
RNA |
| |
|
| Source name |
tumor collected and archived within 30mins after surgery
|
| Organism |
Homo sapiens |
| Characteristics |
Sex:F, age:83
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45 °C on HG U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
|
| Description |
Gene expression data from patients without metastasis
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The baseline was arbitrarily set to 500.
|
| |
|
| Submission date |
Oct 17, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Yi Hong |
| E-mail(s) |
hong.yi@sgh.com.sg, fbap8570@yahoo.com
|
| Organization name |
Singapore General Hospital
|
| Department |
Colorectal Surgery
|
| Lab |
CRC research lab
|
| Street address |
Outram Road
|
| City |
Singapore |
| ZIP/Postal code |
169608 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE9348 |
Expression data from healthy controls and early stage CRC patient's tumor |
|
| Relations |
| Reanalyzed by |
GSE119087 |
