|
| Status |
Public on Sep 08, 2007 |
| Title |
MA69: EC-control-rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
coronary arterial endothelial cells
|
| Organism |
Sus scrofa |
| Characteristics |
farm pig/female/3 months
|
| Treatment protocol |
Cultured endothelial cells for 1 weeks produced cells at passage one (control cells). Serial passaging was performed on a weekly basis until cells at passage four (senescent cells) were reached (test cells). Both control and test cells at basal condition were collected for total RNA extraction for microarray analysis.
|
| Growth protocol |
porcine coronary arterial endothelial cells were brought to primary culture. Cultured endothelial cells for 1 weeks produced cells at passage one (control cells). Serial passaging was performed on a weekly basis until cells at passage four (senescent cells) were reached (test cells).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Rneasy mini kit (Qiagen) performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on GeneChip Porcine Genome Array.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
MA69
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Sep 06, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Mary Lee |
| E-mail(s) |
leemary@hkucc.hku.hk
|
| Phone |
852-28199258
|
| Fax |
852-28170859
|
| Organization name |
The University of Hong Kong
|
| Department |
Dept of Pharmacology
|
| Lab |
L2
|
| Street address |
21 Sassoon Road
|
| City |
Hong Kong |
| ZIP/Postal code |
852 |
| Country |
China |
| |
|
| Platform ID |
GPL3533 |
| Series (1) |
| GSE8974 |
To study the genomic changes in senescent porcine coronary arterial endothelial cells by multiple passaging in vitro |
|
