|
| Status |
Public on Nov 08, 2016 |
| Title |
Day35 Case-derived neural progenitor cells 2 |
| Sample type |
RNA |
| |
|
| Source name |
Day35 Case-derived neural progenitor cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: iPSC-derived neural progenitor
sample source: spinocerebellar ataxia type 6 patient
|
| Growth protocol |
hiPSCs were differentiated in three-dimenstional condition with SFEBq culture.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
iPSC-derived neural progenitors were collected on culture day35, and total RNA was extracted and purified using RNA mini kit (QIAGEN).
|
| Label |
cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 100 ng Total RNA using the One-Color Low Input Quick Amp labeling kit (Agilent:One-Colr) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
0.6 ug of Cy3-labelled cRNA was fragmented at 60°C for 30 minutes in a Agilent fragmentation buffer and was hybridized at 65°C for 17 hr using Gene Expression Hybridization Kit.
|
| Scan protocol |
Slides were scanned on the Agilent SureScan Microarray Scanner (G2600D) after washing with Agilent gene Expression Wash Buffers Pack .
|
| Description |
none
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 11.5.1.1 (Agilent) and Subio Platform (ver. 1.19.4941, Subio Inc). gProcessedSignals were normalized to the 75th percentiles, and transformed into log2 ratio against the average value of normalized intensities of 4 control iPSCs.
|
| |
|
| Submission date |
Aug 09, 2016 |
| Last update date |
Nov 08, 2016 |
| Contact name |
Keiko Muguruma |
| E-mail(s) |
muguruke@hirakata.kmu.ac.jp
|
| Organization name |
Kansai Medical University
|
| Department |
Graduate School of Medicine
|
| Lab |
iPS Cell Applied Medicine
|
| Street address |
2-5-1 Shinmachi
|
| City |
Hirakata |
| ZIP/Postal code |
5731010 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21185 |
| Series (2) |
| GSE85348 |
Vulnerability of Purkinje cells induced from spinocerebellar ataxia type 6 patient-derived iPS cells [2] |
| GSE85349 |
Vulnerability of Purkinje cells induced from spinocerebellar ataxia type 6 patient-derived iPS cells |
|
