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Sample GSM2225006 Query DataSets for GSM2225006
Status Public on Jun 21, 2017
Title CD1c 2
Sample type RNA
 
Source name CD1c dendritic cells (FACS-sorted)
Organism Homo sapiens
Characteristics tissue/cell type: CD1c dendritic cells (FACS-sorted)
molecule subtype: micro RNA
Treatment protocol no specific treatment was performed
Growth protocol none (primary cells were analyzed)
Extracted molecule total RNA
Extraction protocol miRNA was extracted with the miRNeasy FFPE Kit from Qiagen
Label biotin
Label protocol miRNA was labeled with the Affymetrix FlashTag Biotin HSR RNA Labeling Kit
 
Hybridization protocol GeneChip® miRNA 2.0 arrays (Affymetrix) were hybridized with 100ng labelled microRNA, Hybridisation time was 18 hours.
Scan protocol Affymetrix Gene ChIP Scanner 3000 7G
Data processing Statistical analysis was done with the statistical computing environment R. Additional software packages (affy, geneplotter, multtest, and vsn) were taken from the Bioconductor project. Microarray preprocessing: Probe level normalization was conducted using the variance stabilization method (vsn) from Huber et al..
probe group file: UnsupportedGeneChip® miRNA 2.0 Array CDF Files
meta-probeset file: miRNA 2.0 Annotations, Unsupported, CSV format from Affymetrix
 
Submission date Jul 04, 2016
Last update date Jun 21, 2017
Contact name Claudia Döring
E-mail(s) C.Doering@em.uni-frankfurt.de
Organization name Johann Wolfgang-Goethe University Frankfurt a.M.
Department Medical School, Institite of Pathology
Street address Theodor Stern Kai 7
City Frankfurt
ZIP/Postal code 60590
Country Germany
 
Platform ID GPL14613
Series (1)
GSE83994 miRNA expression profiling divides follicular dendritic cell sarcomas into two groups, related to fibroblasts and myopericytomas or Castleman's disease

Data table header descriptions
ID_REF
VALUE log2 vsn normalized gene level expression values from Bioconductor

Data table
ID_REF VALUE
1367452_st 1.913803657
1367453_st 1.909932758
1367454_st 1.287007872
1367455_st 1.385785607
1367456_st 2.931676871
1367457_st 2.801976602
1367458_st 1.594132883
1367459_st 1.948213425
1367460_st 1.602971986
1367461_st 2.803270515
1367462_st 2.697034321
1367463_st 2.137950703
1367464_st 0.942619787
1367465_st 0.490327833
1367466_st 2.399654718
1367467_st 2.962745259
1367468_st 2.232778623
1367469_st 2.046712387
1367470_st 1.379676243
1367471_st 2.102589316

Total number of rows: 20706

Table truncated, full table size 579 Kbytes.




Supplementary file Size Download File type/resource
GSM2225006_CD1c_2_miRNA-2_0_.CEL.gz 542.9 Kb (ftp)(http) CEL
Processed data included within Sample table

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