|
| Status |
Public on Jun 21, 2017 |
| Title |
Castleman2 |
| Sample type |
RNA |
| |
|
| Source name |
microdissected FDCs from Castleman´s disease
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: follicular dendritic cell (FDC) sarcomas
tissue/cell type: microdissected FDCs from Castleman´s disease
molecule subtype: micro RNA
|
| Treatment protocol |
no specific treatment was performed
|
| Growth protocol |
none (primary cells were analyzed)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNA was extracted with the miRNeasy FFPE Kit from Qiagen
|
| Label |
biotin
|
| Label protocol |
miRNA was labeled with the Affymetrix FlashTag Biotin HSR RNA Labeling Kit
|
| |
|
| Hybridization protocol |
GeneChip® miRNA 2.0 arrays (Affymetrix) were hybridized with 100ng labelled microRNA, Hybridisation time was 18 hours.
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Data processing |
Statistical analysis was done with the statistical computing environment R. Additional software packages (affy, geneplotter, multtest, and vsn) were taken from the Bioconductor project. Microarray preprocessing: Probe level normalization was conducted using the variance stabilization method (vsn) from Huber et al..
probe group file: UnsupportedGeneChip® miRNA 2.0 Array CDF Files
meta-probeset file: miRNA 2.0 Annotations, Unsupported, CSV format from Affymetrix
|
| |
|
| Submission date |
Jul 04, 2016 |
| Last update date |
Jun 21, 2017 |
| Contact name |
Claudia Döring |
| E-mail(s) |
C.Doering@em.uni-frankfurt.de
|
| Organization name |
Johann Wolfgang-Goethe University Frankfurt a.M.
|
| Department |
Medical School, Institite of Pathology
|
| Street address |
Theodor Stern Kai 7
|
| City |
Frankfurt |
| ZIP/Postal code |
60590 |
| Country |
Germany |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE83994 |
miRNA expression profiling divides follicular dendritic cell sarcomas into two groups, related to fibroblasts and myopericytomas or Castleman's disease |
|
