|
| Status |
Public on Feb 28, 2017 |
| Title |
MCF7 (CM shEphA2-1) |
| Sample type |
RNA |
| |
|
| Source name |
MCF7 cultured 3 days in the CM prepared from senescent RPE1 expressing shEphA2-1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: breast cancer cell line MCF7
culture protocol: conditioned medium prepared from senescent RPE1 expressing shEphA2-1
|
| Treatment protocol |
Cells were cultured in control 10%FBS/DMEM or CM prepared from doxorubicin-treated RPE1 expressing control or two different EphA2 shRNA.
|
| Growth protocol |
Cells were cultured under atmospheric oxygen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by Trizol Reagent.
|
| Label |
Cy3
|
| Label protocol |
RNA was reverse transcribed, and Cy3-labeled cRNA was generated by in vitro transcription.
|
| |
|
| Hybridization protocol |
Cy3-labeled cRNA was hybridized to Agilent Human Genome 8 x 60 K arrays (Agilent Technologies) according to the manufacturer's instructions.
|
| Scan protocol |
Arrays were scanned by a DNA Microarray Scanner (Agilent).
|
| Data processing |
Microarray images were analyzed with Agilent Feature Extraction software.
Data were normalized to 75th percentile for inter-array comparison.
|
| |
|
| Submission date |
May 12, 2016 |
| Last update date |
Mar 01, 2017 |
| Contact name |
Masaki Takasugi |
| E-mail(s) |
mtjfcr@gmail.com
|
| Organization name |
Osaka Metropolitan University
|
| Department |
Graduate School of Medicine
|
| Street address |
1-4-3 Asahimachi, Abeno-ku
|
| City |
Osaka-shi |
| State/province |
Osaka |
| ZIP/Postal code |
545-8585 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21185 |
| Series (1) |
| GSE81373 |
Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2. |
|
