|
| Status |
Public on Mar 16, 2016 |
| Title |
Infection with Brucella abortus C30 strain for 12 h-2 |
| Sample type |
RNA |
| |
|
| Source name |
RAW 264.7 cells
|
| Organism |
Mus musculus |
| Characteristics |
cell line: RAW 264.7
culture media: RPMI
infection: Brucella abortus C30 strain
time point: 12h
|
| Treatment protocol |
Cells were infected with 10 MOI of Brucella abortus wild and mutant strains for 0, 6, 12, and 24 h.
|
| Growth protocol |
8.0 x 104 cells/cm2 in 6-well plates with 2 mL of RPMI containing 2 % FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the cells using RNeasy Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturers’ protocol. After processing with DNase digestion, clean-up procedures, RNA samples were quantified, aliquot and stored at -80°C until use. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
The sense cDNA was then fragmented and biotin-labeled with TdT (terminal deoxynucleotidyl transferase) using the GeneChip WT Terminal labeling kit.
|
| |
|
| Hybridization protocol |
Approximately 5.5 μg of labeled DNA target was hybridized to the Affymetrix GeneChip Array at 45°C for 16hour
|
| Scan protocol |
Hybridized arrays were washed and stained on a GeneChip Fluidics Station 450 and scanned on a GCS3000 Scanner (Affymetrix).
|
| Description |
Leukemic monocyte macrophage cell line
|
| Data processing |
Array data export processing and analysis was performed using Affymetrix® GeneChip Command Console® Software (AGCC)
|
| |
|
| Submission date |
Mar 15, 2016 |
| Last update date |
Mar 16, 2016 |
| Contact name |
Myunghwan Jung |
| E-mail(s) |
vetmhjung@gmail.com
|
| Phone |
82-2-874-2738
|
| Organization name |
Seoul National University
|
| Department |
Veterinary Medicine
|
| Lab |
Infectious diseases lab
|
| Street address |
1 Gwanak-ro
|
| City |
Seoul |
| ZIP/Postal code |
151-742 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE79264 |
Global gene transcriptional profiles of RAW 264.7 cells following the infection with Brucella abortus wild and mutant strains |
|
