|
| Status |
Public on Oct 30, 2015 |
| Title |
ache_mut_1 |
| Sample type |
SRA |
| |
|
| Source name |
whole larvae
|
| Organism |
Danio rerio |
| Characteristics |
genotype/variation: ache -/-
age: 72hpf
|
| Treatment protocol |
None
|
| Growth protocol |
Zebrafish mutants and wild type siblings grown in standard conditions and medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol total RNA extraction
Stranded TruSeq mRNAseq Illumina
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1000 |
| |
|
| Description |
ache mutant 72hpf
|
| Data processing |
Base calling with RTA v1.13
Alignment with tophat v1.4.1
Quantification made with HTSeq v0.5.3p3
Normalisation and differential expression with DESeq2
Genome_build: zv9
Supplementary_files_format_and_content: Quantification from HTSeq
|
| |
|
| Submission date |
Oct 20, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
olivier ARMANT |
| E-mail(s) |
olivier.armant@kit.edu
|
| Organization name |
Karlsruhe Institute of Technology
|
| Department |
ITG
|
| Lab |
Strähle
|
| Street address |
Hermann-von-Helmholtz-Platz 1
|
| City |
Eggenstein-Leopoldshafen |
| ZIP/Postal code |
D-76344 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16509 |
| Series (1) |
| GSE74202 |
Loss of function of myosin chaperones triggers Hsf1-mediated transcriptional response in skeletal muscle cells |
|
| Relations |
| BioSample |
SAMN04196821 |
| SRA |
SRX1357718 |
