|
| Status |
Public on Oct 14, 2015 |
| Title |
MSU_59b |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
HFD-LFD Late
|
| Organism |
Mus musculus |
| Characteristics |
treatment: DMBA
location: Mammary
tissue: Tumor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
CY5
|
| Label protocol |
Labelled by following Agilent's Low Input Quick Amp Standard Protocol.
|
| |
|
| Channel 2 |
| Source name |
Universal Mouse Reference RNA
|
| Organism |
Mus musculus |
| Characteristics |
content: 11 mouse cell lines
manufacturer: Stratagene
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Labelled by following Agilent's Low Input Quick Amp Standard Protocol.
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Agilent G2505C scanner.
|
| Description |
HFD-LFD Late
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1). Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Oct 13, 2015 |
| Last update date |
Oct 14, 2015 |
| Contact name |
Melissa Troester |
| E-mail(s) |
troester@email.unc.edu
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Center for Environmental Health Sciences Integrated Sciences Facility Corencer Center
|
| Street address |
2104H McGavran-Greenberg 135 Dauer Drive Campus Box 7435 27599-7435
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE73983 |
Puberty-specific promotion of mammary tumorigenesis by a high animal fat diet |
|
