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Sample GSM1886909 Query DataSets for GSM1886909
Status Public on Mar 31, 2016
Title NB BTH+ 12h
Sample type RNA
 
Source name Nipponbare rice+BTH
Organism Oryza sativa
Characteristics genotype/variation: wild type
developmental stage: 3-leaf stage
tissue: seedling
treated with: 30 µM benzothiadiazole (BTH) for 12hrs
Treatment protocol Rice plants at 3-leaf stage were supplied with BTH solutin (30 µM at a final concentration) into the culture medium.
Growth protocol Rice seeds were germinated on MS plates. Seedlings were grown in a plastic box containing 1/1000 hyponex culture medium.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Trizol and cleand up with an RNeasy plant mini kit (Qiagen).
Label Cy3
Label protocol The RNAs (400-ng aliquots) were labeled with a Low RNA Input Linear Amplification/Labeling Kit (Agilent Technologies).
 
Hybridization protocol Agilent protocol G4140-90040
Scan protocol Slides were scanned immediately after washing on Agilent Scanner model G2505B and sofware G2565BA using default parameters.
Description SAMPLE 5
Data processing The scanned imags were analized with Feature Extraction software (version 9.1; Agilent Technologies) using default parameters. The measurements were normalized to their mean.
 
Submission date Sep 17, 2015
Last update date Mar 31, 2016
Contact name Hiroshi Takatsuji
E-mail(s) takatsuh@affrc.go.jp
Organization name National Institute of Agrobiological Sciences
Department Division of Plant Sciences
Lab Plant Disease Resistance Research Unit
Street address 2-1-2 Kannondai
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8602
Country Japan
 
Platform ID GPL8852
Series (1)
GSE73113 The BTH-incuced WRKY62-dependent genes

Data table header descriptions
ID_REF
VALUE log2 normalized

Data table
ID_REF VALUE
Os03g0146300|mRNA|AK121395|CDS+3'UTR 0.27939
Os03g0100100|COMBINER|CI224620|6 -0.53293
Os06g0591200|mRNA|AK062563|CDS+3'UTR 0.38891
Os01g0658400|mRNA|AK099284|CDS+3'UTR 0.18933
Os01g0388500|COMBINER_EST|Os01g0388500|8 0.16155
Os12g0403800|mRNA|AK059940|CDS+3'UTR 0.47081
Os07g0572000|mRNA|AK111653|CDS+3'UTR 0.07990
Os06g0530200|mRNA|AK073116|CDS+3'UTR 0.00962
Os02g0149500|mRNA|AK109901|CDS+3'UTR 0.10004
Os02g0187300|mRNA|AK062633|UTR -0.45176
Os09g0546900|mRNA|AK062365|CDS+3'UTR -0.29545
Os04g0308100|mRNA|AK108495|CDS+3'UTR 0.57782
Os03g0194500|COMBINER_EST|CI391251|0 -0.20709
Os08g0555800|COMBINER_EST|Os08g0555800|8 -0.17701
Os10g0522900|mRNA|AK068314|CDS+3'UTR 0.02462
Os01g0871100|mRNA|AK064743|CDS+3'UTR 0.04126
Os06g0561800|COMBINER_EST|Os06g0561800|8 -0.12404
Os03g0684000|mRNA|AK105025|CDS+3'UTR -0.07136
Os07g0664600|mRNA|AK105211|CDS+3'UTR 1.54158
Os04g0484300|COMBINER|CI434770|6 -0.13620

Total number of rows: 42537

Table truncated, full table size 1879 Kbytes.




Supplementary file Size Download File type/resource
GSM1886909_NB_BTH+_12h.txt.gz 7.7 Mb (ftp)(http) TXT
Processed data included within Sample table
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