|
| Status |
Public on Nov 04, 2015 |
| Title |
BMS-Akt1 CC-Alone 3 |
| Sample type |
SRA |
| |
|
| Source name |
Bone marrow stroma, non-co-cultured
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6J
cell type: bone marrow stroma
co-culture: alone
sample type: in vitro
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol treatment.
Illumina TruSeq PE 51x2 (RNA-seq).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
PolyA RNA
|
| Data processing |
Illumina Casava1.8 software used for basecalling.
RNA-seq: Sequence reads were aligned to mm9 using TopHat with default parameters.
RNA-seq: FPKM values were obtained using CuffLinks with upper-quartile and GC normalization.
Genome_build: MGSCv37 (mm9)
Supplementary_files_format_and_content: RNA-seq: Tab-delimited text files include FPKM values for each RefSeq gene.
|
| |
|
| Submission date |
Aug 20, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Olivier Elemento |
| E-mail(s) |
ole2001@med.cornell.edu
|
| Organization name |
WEILL MEDICAL COLLEGE OF CORNELL UNIV
|
| Street address |
1305 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE61636 |
Isolation and functional characterization of the arteriole hematopoietic stem cell niche |
|
| Relations |
| BioSample |
SAMN04004071 |
| SRA |
SRX1161225 |
