|
| Status |
Public on Aug 04, 2015 |
| Title |
Serum miRNA from chronic daily male ethanol rat replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Rat blood serum
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Long Evans Hooded rats
tissue: blood serum
treatment age onset: adolescent
Sex: male
animal pair: 23
exposure paradigm: daily
|
| Treatment protocol |
At postnatal day 29, Long Evans Hooded rats were split into four groups (two treatment and two control groups). Two groups of rats were divided into pairs based on gender and body weight and weaned onto an ad lib liquid ethanol (6.7%)-containing diet or an isolcaloric liquid control diet (containing maltose instead of ethanol) for 3 weeks. The other two groups of rats began receiving the same liquid ethanol or control diet in young adulthood (postnatal day 79) using a 3 week episodic binge-drinking paradigm (3 days liquid ethanol or control diet followed by 4 days ad lib solid chow). After the three week ethanol exposure period, all rats in each group were euthanized with CO2 and blood was collected into BD Vacutainer® SST™ tubes for serum miRNA isolation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen total RNA
|
| Label |
biotin
|
| Label protocol |
Affymetrix standard 1 cycle
|
| |
|
| Hybridization protocol |
Affymetrix EukGE-WS2v4 Protocol
|
| Scan protocol |
Standard Affymetrix Genechip 7G Scanner
|
| Description |
miRNA expression data from rat blood serum
|
| Data processing |
RMA analysis using Affymetrix Expression Console software
|
| |
|
| Submission date |
Aug 03, 2015 |
| Last update date |
Aug 04, 2015 |
| Contact name |
Frank A Middleton |
| E-mail(s) |
middletf@upstate.edu
|
| Phone |
315-464-7721
|
| Organization name |
SUNY Upstate Medical Univ
|
| Department |
Neuroscience and Physiology
|
| Street address |
505 Irving Avenue
|
| City |
Syracuse |
| State/province |
NY |
| ZIP/Postal code |
13210 |
| Country |
USA |
| |
|
| Platform ID |
GPL14613 |
| Series (2) |
| GSE71579 |
Alterations in serum microRNA in humans with alcohol use disorders impact cell proliferation and cell death pathways and predict structural and functional changes in brain |
| GSE71653 |
Alterations in serum microRNA in humans with alcohol use disorders impact cell proliferation and cell death pathways and predict structural and functional changes in brain [rat miRNA 2.0] |
|
