|
| Status |
Public on Aug 04, 2015 |
| Title |
Mouse NSC treated with TGF replicate 5 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse NS5 neural stem cells
|
| Organism |
Mus musculus |
| Characteristics |
cell line: NS5
cell type: neural stem cells
media supplement: TGF beta 1
exposure: 10 mg/ml TGF beta 1
|
| Treatment protocol |
Plates were separated into 4 groups and exposed to different media for 48 hours at 37C: maintenance media supplemented with 10 mg/ml FGF2 (Preprotech) or TGFβ1 (R&D Systems). In another set of dishes, 400 mg/dl of ethanol was added in each type of supplemented media. All dishes were placed inside airtight containers with a small container of either sterile water or 400 mg/dl ethanol to maintain constant concentration throughout the exposure period.
|
| Growth protocol |
A total of eighteen 10 cm dishes coated with poly-L-ornithine hydrobromide and laminin (Sigma) were plated with 3 million cells each and incubated at 37C for 24 hours in a Euromed-N maintenance medium (Euroclone).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen total RNA
|
| Label |
biotin
|
| Label protocol |
Affymetrix standard 1 cycle
|
| |
|
| Hybridization protocol |
Affymetrix EukGE-WS2v4 Protocol
|
| Scan protocol |
Standard Affymetrix Genechip 7G Scanner
|
| Description |
miRNA expression data from mouse neural stem cells
|
| Data processing |
RMA analysis using Affymetrix Expression Console software
|
| |
|
| Submission date |
Aug 03, 2015 |
| Last update date |
Aug 04, 2015 |
| Contact name |
Frank A Middleton |
| E-mail(s) |
middletf@upstate.edu
|
| Phone |
315-464-7721
|
| Organization name |
SUNY Upstate Medical Univ
|
| Department |
Neuroscience and Physiology
|
| Street address |
505 Irving Avenue
|
| City |
Syracuse |
| State/province |
NY |
| ZIP/Postal code |
13210 |
| Country |
USA |
| |
|
| Platform ID |
GPL14613 |
| Series (2) |
| GSE71579 |
Alterations in serum microRNA in humans with alcohol use disorders impact cell proliferation and cell death pathways and predict structural and functional changes in brain |
| GSE71652 |
Alterations in serum microRNA in humans with alcohol use disorders impact cell proliferation and cell death pathways and predict structural and functional changes in brain [mouse NSC miRNA 2.0] |
|
