|
| Status |
Public on Sep 30, 2016 |
| Title |
30_Leaves_Sangiovese_Stress 1_Sampling2 |
| Sample type |
RNA |
| |
|
| Source name |
Sangiovese leaves collected six days after water deprivation, replicate 3
|
| Organism |
Vitis vinifera |
| Characteristics |
cultivar: Sangiovese
tissue: Leaves
treatment: Water stress
sampling: Sampling 2, Six days after water deprivation
|
| Treatment protocol |
Ten vines per cultivar were used and maintained at about 90% of maximum water availability (WW, well watered vines) and 10 vines received, from fruit-set to veraison, 40% of maximum water availability (WS, water stressed vines) (Figure 1). During water limitation, all stressed vines were covered with a plastic film to avoid interference due to rainfall and soil water evaporation. Re-watering was performed 46 days after stress imposition and the post-recovery plant material (ex-WS) was collected after 24 days (i.e. 70 days after the onset of water stress).
|
| Growth protocol |
This study was conducted in 2011 on eight-year-old potted (60 L) vines of cv. Sangiovese (clone VCR30) and cv. Montepulciano (clone R7) grafted onto 1103 Paulsen rootstock and grown in an outdoor area close to the Faculty of Agriculture of the University of Perugia (Region of Umbria, central Italy, 42°58’ N, 12°24’ E, altitude 405 m a.s.l.).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from ~50 mg of frozen leaves and ~200 mg of berry (pericarp plus seeds) using the Spectrum™ Plant Total RNA kit (Sigma-Aldrich, St. Louis, MO) with some modifications as reported in Fasoli, et al. (2012). RNA quality and quantity were assessed using a Nanodrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE) and a Bioanalyzer Chip RNA 7500 series II (Agilent, Santa Clara, CA).
|
| Label |
Cy3
|
| Label protocol |
cDNA synthesis and labeling reactions were performed according to the NimbleGen Arrays User’s Guide (V 3.2).
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| |
|
| Hybridization protocol |
Hybridization and washing procedures were performed according to the NimbleGen Arrays User’s Guide (V 3.2).
|
| Scan protocol |
Each microarray was scanned using a Axon GenePix 4400 A at 532 nm (Cy-3 absorption peak) and GenePix Pro7 software (Molecular Devices, Sunnyvale, CA, USA) according to the manufacturers' instructions.
|
| Data processing |
Images were analyzed using NimbleScan v2.5 software (Roche), background correction and standard RMA normalization were selected.
|
| |
|
| Submission date |
Jul 09, 2015 |
| Last update date |
Sep 30, 2016 |
| Contact name |
Marianna Fasoli |
| E-mail(s) |
marianna.fasoli@univr.it
|
| Phone |
+39 045 842 5625
|
| Organization name |
University of Verona
|
| Department |
Biotechnology
|
| Lab |
Molecular Viticulture
|
| Street address |
Via della Pieve 70
|
| City |
San Pietro in Cariano |
| State/province |
Verona |
| ZIP/Postal code |
37029 |
| Country |
Italy |
| |
|
| Platform ID |
GPL17894 |
| Series (1) |
| GSE70670 |
Distinct transcriptome responses to water limitation in isohydric and anisohydric grapevine cultivars |
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