|
| Status |
Public on Aug 03, 2015 |
| Title |
Placenta_GDM_Yes_ID55_expression |
| Sample type |
RNA |
| |
|
| Source name |
Placenta tissue from the maternal (decidual basalis) side near the umbilical cord
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: placenta
gestational diabetes: Yes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the mirVANA RNA Isolation Kit (Ambion Inc., Austin, TX)
|
| Label |
biotin
|
| Label protocol |
Affymetrix's GeneChip WT PLUS with the WT Terminal Labeling Kit
|
| |
|
| Hybridization protocol |
50 ng of high-quality RNA (260/280=1.8-2.0; RIN>6.0) was used to generate sense-strand cDNA with the Ambion® WT Expression Kit (Life Technologies), and then hybridised to the Affymetrix GeneChip® Human Transcriptome Array 2.0 using standard Affymetrix protocol
|
| Scan protocol |
Arrays were scanned using the Affymetrix GeneChip Scanner
|
| Description |
pregnancy without maternal complications
|
| Data processing |
CEL files containing the measured expression intensities were processed using the Affymetrix Expression Console Software. Background correction was performed using Robust Multichip Analysis (RMA), probe values were then quantile normalized, and summarized into one gene-level expression measure using median polish (Expression Console Software Default Setting).
|
| |
|
| Submission date |
Jul 02, 2015 |
| Last update date |
Aug 03, 2015 |
| Contact name |
Alexandra Binder |
| Organization name |
University of California Los Angeles
|
| Department |
Department of Epidemiology
|
| Street address |
650 Charles Young Drive, South
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL17586 |
| Series (2) |
| GSE70493 |
Epigenome-wide and Transcriptome-wide Analyses Reveal Gestational Diabetes is Associated with Alterations in the Human Leukocyte Antigen Complex [gene expression] |
| GSE70494 |
Epigenome-wide and Transcriptome-wide Analyses Reveal Gestational Diabetes is Associated with Alterations in the Human Leukocyte Antigen Complex |
|
