|
| Status |
Public on Apr 11, 2017 |
| Title |
CD14 SMM2 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow CD14+ cells from SMM patient 2
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: bone marrow
age: 72 years
|
| Treatment protocol |
No treatment was applied to CD14+ cells.
|
| Growth protocol |
CD14+ cells from bone marrow of 8 MGUS, 11 SMM and 16 MM patients. The CD14+ cell fraction was obtained by immunomagnetic cells sorting using anti-CD14 monoclonal antibody-coated microbeads (MACS, Miltenyi Biotec., Bergish-Gladbach, Germany). Samples with a purity of more than 90%, tested via flow-cytometry (BD biosciences, San Josè, CA) were analyzed. These samples were evaluated immediately after purification.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from CD14+ cells with Rneasy Mini Kit (Qiagen, Valencia, CA)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms of total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 micrograms of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
HG-U133 2.0 Plus arrays were scanned using the Scanner G3000 7G (Affymetrix).
|
| Description |
Gene expression profiling data of CD14+ cells from bone marrow of SMM patient 2
|
| Data processing |
The probe-level signals were converted to expression values using the Bioconductor function for Robust Multi-array Analysis (RMA), in which perfect match intensities are background adjusted, normalized by means of quantile-quantile normalization and log2-transformed.
|
| |
|
| Submission date |
Jun 26, 2015 |
| Last update date |
Apr 11, 2017 |
| Contact name |
Luca Agnelli |
| E-mail(s) |
luca.agnelli@istitutotumori.mi.it, luca.agnelli@gmail.com
|
| Phone |
+390223903581
|
| Organization name |
IRCCS Istituto Nazionale dei Tumori
|
| Department |
Department of Advanced Diagnostics
|
| Street address |
Venezian 1
|
| City |
MILAN |
| ZIP/Postal code |
20133 |
| Country |
Italy |
| |
|
| Platform ID |
GPL22321 |
| Series (1) |
| GSE70345 |
IL21R-expressing CD14+CD16+ monocytes expand in multiple myeloma patients leading to increased osteoclasts |
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