|
| Status |
Public on Nov 17, 2015 |
| Title |
Old SAMP8 2 |
| Sample type |
SRA |
| |
|
| Source name |
Brain hippocampus
|
| Organism |
Mus musculus |
| Characteristics |
strain: SAMP8/TaHsd
tissue: Brain
age: ten months old
treatment: control
|
| Treatment protocol |
Mice were fed control diet and diet containing J147
|
| Growth protocol |
All experiments were performed in accordance with the US Public Health Service Guide for Care and Use of Laboratory Animals and protocols approved by the IACUC at the Salk Institute.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was isolated using the RNeasy Plus Universal mini kit (Qiagen)
Illumina TruSeq Stranded mRNA
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
alignment with STAR 2.3.0e
gene-level read counts with featureCounts 1.4.6 (Subread package)
Genome_build: mm9
Supplementary_files_format_and_content: per-gene raw read count (CSV)
|
| |
|
| Submission date |
May 26, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Max Chang |
| E-mail(s) |
mchang@ucsd.edu
|
| Organization name |
University of California, San Diego
|
| Street address |
9500 Gilman Drive
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE69244 |
Whole transcriptome analysis of brain hippocampal tissue from young and old senescence-accelerated prone SAMP8 mice treated with control diet and J147-containing diet |
|
| Relations |
| BioSample |
SAMN03734794 |
| SRA |
SRX1038776 |
