|
| Status |
Public on Feb 27, 2018 |
| Title |
piglet pig milk replacer rep7 |
| Sample type |
RNA |
| |
|
| Source name |
piglet retina, pig milk replacer, postnatal day 38
|
| Organism |
Sus scrofa |
| Characteristics |
strain: Domestic pig (Landrace x Large White)
gender: male
tissue: retina
age: postnatal day 38
treatment group: control group feeding basal level
|
| Treatment protocol |
Pig retina samples were collected after sacrifice and immediately stored in liquid nitrogen tank until RNA extraction.
|
| Growth protocol |
Three-day-old male domestic piglets (Sus scrofa, Landrace×Large White cross) were purchased from a commercial piggery in Xiamen City, China and randomly placed in the 2 groups. All animals were kept in the temperature-controlled environment with a 12-h light (08:00~20:00) and a 12-h dark (20:00~08:00) cycle. The home pens contained a “nest” (a rubber tire covered with a clean towel), a heat lamp over the nest and an identical woody toy hang in the home pen. All facilities (including home pens and behavior room) were monitored for 24-h. The feeding time was fixed as 8:00, 13:00, 18:00, 22:30, with an extra 50mL milk/pig supplied at the last feeding.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The extraction of total RNA was performed using Agencourt RNAdvance tissue kit according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45˚C on Affymetrix Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneArray Scanner G3000 7G system.
|
| Description |
Gene expression data from piglet retina of pig milk replacer
|
| Data processing |
The data were analyzed with Expression console software using Robust Multichip Average(RMA) as normalization method.
|
| |
|
| Submission date |
Feb 27, 2015 |
| Last update date |
Feb 27, 2018 |
| Contact name |
yujie shi |
| E-mail(s) |
yujie.shi@rd.nestle.com
|
| Phone |
+8613426001743
|
| Organization name |
Nestle research center- Beijing hub
|
| Street address |
No.5 building No.5 Road Dijin
|
| City |
Beijing |
| ZIP/Postal code |
100095 |
| Country |
China |
| |
|
| Platform ID |
GPL3533 |
| Series (1) |
| GSE66363 |
Expression data from postnatal piglet retina, investigating how sialyllactose benefit retina development and vision |
|
