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Sample GSM1617930 Query DataSets for GSM1617930
Status Public on Sep 08, 2016
Title A549_COMB_3
Sample type RNA
 
Source name Lung
Organism Homo sapiens
Characteristics individual: Diseased donor
cell line: NSCLC cell line A549
cell line type: BRG1 mutant
treatment: COMB
Treatment protocol GCRA: Dexamethasone (1uM) + Retinoic Acid (1uM) 120hours; COMB: Dexamethasone (1uM) + Retinoic Acid (1uM) + Aza (0.2uM) + SAHA (0.2uM) 120 hours
Growth protocol Cells where growth on RPMI media with 10% FBS, 1% PS
Extracted molecule total RNA
Extraction protocol Total RNA were extracted using Trizol Reagent.
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 45ng total RNA using a LowInput QuickAmp Labeling Kit (Agilent Technologies, Palo Alto, Calif, USA) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
 
Hybridization protocol 600 ng of Cy3-labelled cRNA (specific activity >6 pmol Cy3/ug cRNA) was fragmented at 60°C for 30 minutes in a total volume of 25ul following the manufacturers instructions. On completion of the fragmentation reaction, 25ul of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent Whole Human Genome Oligo Microarrays (G4851A) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.
Scan protocol Scanner Agilent G2565CA Feature extraction 10.7
Description Sample name: A549_P_COMB_3043
Cancer cell line
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent). Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
 
Submission date Feb 24, 2015
Last update date Apr 23, 2018
Contact name Manel Esteller
Organization name IDIBELL
Department PEBC
Lab Cancer Epigenetics
Street address Hospital Duran i Reynals Av. Gran Via s/n km, 2.7
City L'Hospitalet de Llobregat
State/province Barcelona
ZIP/Postal code 08908
Country Spain
 
Platform ID GPL17077
Series (2)
GSE66244 Identification of expresion patterns after treatment with GC/RA and combination with AZA/SAHA in MYC amplified and BRG1 mutant lung cancer cell lines
GSE66245 Treatment with GC/RA and combination with AZA/SAHA in MYC amplified and BRG1 mutant lung cancer cell lines
Relations
Reanalyzed by GSE113533

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 16.85253112
DarkCorner 4.734493059
A_23_P117082 12.70597792
A_33_P3246448 5.480436984
A_33_P3318220 4.697447124
A_33_P3236322 5.424429647
A_33_P3319925 5.275953521
A_21_P0000509 18.38002159
A_21_P0000744 9.90275848
A_24_P215804 7.634842527
A_23_P110167 12.4294702
A_33_P3211513 8.364651099
A_23_P103349 4.8551527
A_32_P61480 4.950606969
A_33_P3788124 5.366477717
A_33_P3414202 7.291147839
A_33_P3316686 9.081918584
A_33_P3300975 10.05773305
A_33_P3263061 12.75280252
A_33_P3261373 4.697447124

Total number of rows: 50739

Table truncated, full table size 1259 Kbytes.




Supplementary file Size Download File type/resource
GSM1617930_454_NA_1200253949431324_Cy3_3043_1_2.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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