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Sample GSM1614094 Query DataSets for GSM1614094
Status Public on Sep 08, 2016
Title H460_shBRG1_FBS
Sample type genomic
 
Source name Lung
Organism Homo sapiens
Characteristics individual: Diseased donor
cell line: NSCLC cell line H460
cell line type: MYC amplified
treatment: shBRG1_FBS
Treatment protocol GCRA: Dexamethasone (1uM) + Retinoic Acid (1uM) 120hours; COMB: Dexamethasone (1uM) + Retinoic Acid (1uM) + Aza (0.2uM) + SAHA (0.2uM) 120 hours. shRNA targeting BRG1 (an ATPase of the SWI/SNF Chromatin Remodeling Complex).
Growth protocol Cells where growth on RPMI media with 10% FBS, 1% PS
Extracted molecule genomic DNA
Extraction protocol Total DNA where extrated using Trizol Reagent.
Label Cy3, Cy5
Label protocol Standard Illumina labelling protocol
 
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using High Scan SQ using standard recommended Illumina scanner setting
Description Sample name: h460shFBS
Cancer cell line
Data processing Genome Studio V2011.2
 
Submission date Feb 19, 2015
Last update date Sep 08, 2016
Contact name Manel Esteller
Organization name IDIBELL
Department PEBC
Lab Cancer Epigenetics
Street address Hospital Duran i Reynals Av. Gran Via s/n km, 2.7
City L'Hospitalet de Llobregat
State/province Barcelona
ZIP/Postal code 08908
Country Spain
 
Platform ID GPL13534
Series (2)
GSE66087 Patterns of changes in DNA global methylation following the treatment with GC/RA and with GC/RA plus AZA/SAHA in MYC amplified and BRG1 mutant lung cancer cell lines
GSE66245 Treatment with GC/RA and combination with AZA/SAHA in MYC amplified and BRG1 mutant lung cancer cell lines

Data table header descriptions
ID_REF
VALUE Average Beta
DETECTION P-VALUE

Data table
ID_REF VALUE DETECTION P-VALUE
cg00050873 0.508244271309314 0
cg00212031 0.982740943688245 0
cg00213748 0.0557692873184681 0
cg00214611 0 0
cg00455876 0.600673777622026 0
cg01707559 0.184997130713079 0
cg02004872 0.00834025492323287 0
cg02011394 0.820271626572679 0
cg02050847 0.93248237871367 0
cg02233190 0.012835854466699 0
cg02494853 0.203849179824998 0
cg02839557 0.0624985810575673 0
cg02842889 0.00559639618111581 0
cg03052502 0.937549140626468 0
cg03155755 0.828571645028698 0
cg03244189 0.0848544316064194 0
cg03443143 0.655687035790303 0
cg03683899 0.0067450420848161 0
cg03695421 0.540962119729347 0
cg03706273 0.456955482111629 0

Total number of rows: 485512

Table truncated, full table size 14782 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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