|
| Status |
Public on Sep 08, 2016 |
| Title |
H82_shBRG1_GCRA |
| Sample type |
genomic |
| |
|
| Source name |
Lung
|
| Organism |
Homo sapiens |
| Characteristics |
individual: Diseased donor
cell line: SCLC cell line H82
cell line type: MYC amplified
treatment: shBRG1_GCRA
|
| Treatment protocol |
GCRA: Dexamethasone (1uM) + Retinoic Acid (1uM) 120hours; COMB: Dexamethasone (1uM) + Retinoic Acid (1uM) + Aza (0.2uM) + SAHA (0.2uM) 120 hours. shRNA targeting BRG1 (an ATPase of the SWI/SNF Chromatin Remodeling Complex).
|
| Growth protocol |
Cells where growth on RPMI media with 10% FBS, 1% PS
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA where extrated using Trizol Reagent.
|
| Label |
Cy3, Cy5
|
| Label protocol |
Standard Illumina labelling protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using High Scan SQ using standard recommended Illumina scanner setting
|
| Description |
Sample name: h82shGCRA
Cancer cell line
|
| Data processing |
Genome Studio V2011.2
|
| |
|
| Submission date |
Feb 19, 2015 |
| Last update date |
Sep 08, 2016 |
| Contact name |
Manel Esteller |
| Organization name |
IDIBELL
|
| Department |
PEBC
|
| Lab |
Cancer Epigenetics
|
| Street address |
Hospital Duran i Reynals Av. Gran Via s/n km, 2.7
|
| City |
L'Hospitalet de Llobregat |
| State/province |
Barcelona |
| ZIP/Postal code |
08908 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE66087 |
Patterns of changes in DNA global methylation following the treatment with GC/RA and with GC/RA plus AZA/SAHA in MYC amplified and BRG1 mutant lung cancer cell lines |
| GSE66245 |
Treatment with GC/RA and combination with AZA/SAHA in MYC amplified and BRG1 mutant lung cancer cell lines |
|
