|
Status |
Public on Sep 13, 2016 |
Title |
nonviral 4 factor stromal primed cord blood iPSC clone LZ6+10, passage 13 |
Sample type |
genomic |
|
|
Source name |
nonviral 4 factor (SOX2, OCT4, KLF4, MYC) stromal primed cord blood iPSC clone LZ6+10, passage 13
|
Organism |
Homo sapiens |
Characteristics |
cell type: nonviral 4 factor (SOX2, OCT4, KLF4, MYC) stromal primed cord blood iPSC clone LZ6+10, passage 13 sample type: non-integrated stromal primed cord blood iPSC generated in the Zambidis laboratory
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Phenol-chloroform extraction.
|
Label |
Cy5, Cy3
|
Label protocol |
Standard Illumina protocol.
|
|
|
Hybridization protocol |
Bisulphite-converted (EZ DNA methylation kit) DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using the standard Illumina protocol.
|
Scan protocol |
Arrays were imaged using the BeadArray Reader using the standard recommended Illumina scanner setting.
|
Description |
Individual sample
|
Data processing |
Data were extracted with Methylation Module in GenomeStudio to obtain signal intensities from multiple chips comprising this single series.
|
|
|
Submission date |
Jan 22, 2015 |
Last update date |
Sep 13, 2016 |
Contact name |
Elias Thomas Zambidis |
Organization name |
Johns Hopkins School of Medicine
|
Department |
Oncology
|
Street address |
733 N. Broadway
|
City |
Baltimore |
State/province |
MD |
ZIP/Postal code |
21205 |
Country |
USA |
|
|
Platform ID |
GPL13534 |
Series (2) |
GSE44430 |
Comprehensive microarray analysis of reprogramming fidelity in a library of non-integrated induced pluripotent stem cell lines prepared with various derivation methods |
GSE65214 |
Global DNA CpG methylation profiling of human embryonic stem cells, fibroblast iPSC, and low passage stromal primed myeloid iPSC before and after conversion to the naïve state |
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