nonviral 7F fetal fibroblast iPSC clone 6.1, passage 38, p7 after conversion to a naïve state with L3i
Sample type
genomic
Source name
nonviral 7 factor (SOX2, OCT4, KLF4, MYC, LIN28, NANOG, SV40 T antigen) fetal Fibroblast iPSC clone 6.1, passage 38, p7 after conversion to a naïve state with L3i
cell type: nonviral 7 factor (SOX2, OCT4, KLF4, MYC, LIN28, NANOG, SV40 T antigen) fetal Fibroblast iPSC clone 6.1, passage 38, p7 after conversion to a naïve state with L3i sample type: non-integrated fetal Fibroblast iPSC generated in the Zambidis laboratory p7 after conversion to a naïve state with L3i
Extracted molecule
genomic DNA
Extraction protocol
Phenol-chloroform extraction.
Label
Cy5, Cy3
Label protocol
Standard Illumina protocol.
Hybridization protocol
Bisulphite-converted (EZ DNA methylation kit) DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using the standard Illumina protocol.
Scan protocol
Arrays were imaged using the BeadArray Reader using the standard recommended Illumina scanner setting.
Description
Individual sample
Data processing
Data were extracted with Methylation Module in GenomeStudio to obtain signal intensities from multiple chips comprising this single series.
Comprehensive microarray analysis of reprogramming fidelity in a library of non-integrated induced pluripotent stem cell lines prepared with various derivation methods
Global DNA CpG methylation profiling of human embryonic stem cells, fibroblast iPSC, and low passage stromal primed myeloid iPSC before and after conversion to the naïve state
