cell type: nonviral 4 factor (SOX2, OCT4, KLF4, MYC) stromal primed cord blood iPSC clone LZ6+2, passage 17, p4 after conversion to a naïve state with L3i sample type: non-integrated stromal primed cord blood iPSC generated in the Zambidis laboratory, p4 after conversion to a naïve state with L3i
Extracted molecule
genomic DNA
Extraction protocol
Phenol-chloroform extraction.
Label
Cy5, Cy3
Label protocol
Standard Illumina protocol.
Hybridization protocol
Bisulphite-converted (EZ DNA methylation kit) DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using the standard Illumina protocol.
Scan protocol
Arrays were imaged using the BeadArray Reader using the standard recommended Illumina scanner setting.
Description
Individual sample
Data processing
Data were extracted with Methylation Module in GenomeStudio to obtain signal intensities from multiple chips comprising this single series.
Comprehensive microarray analysis of reprogramming fidelity in a library of non-integrated induced pluripotent stem cell lines prepared with various derivation methods
Global DNA CpG methylation profiling of human embryonic stem cells, fibroblast iPSC, and low passage stromal primed myeloid iPSC before and after conversion to the naïve state