|
| Status |
Public on Sep 13, 2016 |
| Title |
nonviral 7F stromal primed cord blood iPSC clone 6.2 passage 26, p7 after conversion to a naïve state with L3i |
| Sample type |
genomic |
| |
|
| Source name |
nonviral 7F (SOX2, OCT4, KLF4, MYC, LIN28, NANOG, SV40 T antigen) stromal primed cord blood iPSC clone 6.2 passage 26, p7 after conversion to a naïve state with L3i
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: nonviral 7F stromal primed cord blood iPSC clone 6.2 passage 26, p7 after conversion to a naïve state with L3i
sample type: non-integrated stromal primed cord blood iPSC generated in the Zambidis laboratory, p7 after conversion to a naïve state with L3i
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Phenol-chloroform extraction.
|
| Label |
Cy5, Cy3
|
| Label protocol |
Standard Illumina protocol.
|
| |
|
| Hybridization protocol |
Bisulphite-converted (EZ DNA methylation kit) DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using the standard Illumina protocol.
|
| Scan protocol |
Arrays were imaged using the BeadArray Reader using the standard recommended Illumina scanner setting.
|
| Description |
Individual sample
|
| Data processing |
Data were extracted with Methylation Module in GenomeStudio to obtain signal intensities from multiple chips comprising this single series.
|
| |
|
| Submission date |
Jan 22, 2015 |
| Last update date |
Sep 13, 2016 |
| Contact name |
Elias Thomas Zambidis |
| Organization name |
Johns Hopkins School of Medicine
|
| Department |
Oncology
|
| Street address |
733 N. Broadway
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE44430 |
Comprehensive microarray analysis of reprogramming fidelity in a library of non-integrated induced pluripotent stem cell lines prepared with various derivation methods |
| GSE65214 |
Global DNA CpG methylation profiling of human embryonic stem cells, fibroblast iPSC, and low passage stromal primed myeloid iPSC before and after conversion to the naïve state |
|
