|
| Status |
Public on Sep 30, 2015 |
| Title |
35-day-old suckling piglet replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
age of 35days suckling piglet
|
| Organism |
Sus scrofa |
| Characteristics |
tissue: ileal mucosa
age: 35day
weaning: no
|
| Treatment protocol |
Collected ileal mucosa was immersed into RNAlate. The samples were kept at 4°C overnight and then stored at −80°C until use.
|
| Growth protocol |
All animals were crossbred (Landrace × Large white × Duroc) piglets and were bred at a commercial farm in Japan. Weaning piglets received a typical commercial diet for weaning.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
leal tissue (75% in length from the pyloric sphincter) was excised, processed in ice-cold PBS, transferred to RNAlater (Ambion) and stored at -20 °C. Tissue was removed from RNAlater, lyzed in Trizol (Invitrogen), and RNA was extracted using chloroform/isopropanol steps. Total RNA was further treated with the RNeasy kit (Qiagen) according to the manufacturer’s instructions, including an RNase-free DNase I (Qiagen) digestion step.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (GeneChip 3'IVT Express Kit, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Porcine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from porcine ileal mucosa
S35D-3
|
| Data processing |
Initial data analysis was performed using Affymetrix GCOS 1.2 software. Data were further analyzed using Bioconductor (http://www.bioconductor.org) from the R Project for Statistical Computing (http://www.r-project.org). Normalization was performed with gcRMA.
|
| |
|
| Submission date |
Jan 15, 2015 |
| Last update date |
Sep 30, 2015 |
| Contact name |
Ryo Inoue |
| E-mail(s) |
r-inoue@kpu.ac.jp
|
| Organization name |
Kyoto Prefectural University
|
| Street address |
1-5, Hangi-cho, shimogamo
|
| City |
Kyoto |
| ZIP/Postal code |
606-8522 |
| Country |
Japan |
| |
|
| Platform ID |
GPL3533 |
| Series (1) |
| GSE65008 |
Expression data of ileal mucosa in developing piglets |
|
