|
Status |
Public on Jun 03, 2015 |
Title |
CRBM of subject 448 (Sample128) |
Sample type |
genomic |
|
|
Source name |
Brain CRBM
|
Organism |
Homo sapiens |
Characteristics |
tissue: Brain CRBM subject id: 448 Sex: M predicted gender: male age: 85 dna methylation age: 53.6244769231651 age acceleration with respect to noncerebellar samples: -12.6492782908713 cor sample vs gold standard: 0.836631529085048 mean abs difference sample vs gold standard: 0.0976103561501273 postmortem interval: 36
|
Growth protocol |
DNA extracted from fresh frozen tissue
|
Extracted molecule |
genomic DNA |
Extraction protocol |
genomic DNA was extracted and purified from fresh frozen tissue using the AllPrep DNA/RNA mini kit according to standard instructions.
|
Label |
Cy3 and Cy5
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
Data processing |
BeadStudio software v3.2. Unmethylated and methylated signal intensities and detection p values included in Series supplementary file
|
|
|
Submission date |
Dec 24, 2014 |
Last update date |
Jun 03, 2015 |
Contact name |
Steve Horvath |
E-mail(s) |
shorvath@altoslabs.com
|
Organization name |
University of California, Los Angeles
|
Department |
Human Genetics
|
Lab |
Horvath
|
Street address |
695 Charles E. Young Drive South, Box 708822
|
City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90095-7088 |
Country |
USA |
|
|
Platform ID |
GPL13534 |
Series (2) |
GSE64509 |
DNA methylation profiles of human brain tissue samples |
GSE64511 |
DNA methylation profiles of multiple human tissues |
|