|
| Status |
Public on Jun 03, 2015 |
| Title |
genomic DNA from bone of subject 33 |
| Sample type |
genomic |
| |
|
| Source name |
Bone
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: bone
subject id: 33
Sex: female
sexpredictedusingdnamethdata: female
age: 77
dna methylation age: 75.3247377110313
|
| Growth protocol |
DNA extracted from fresh frozen bone
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted and purified from fresh frozen tissue using AllPrep DNA/RNA mini kit according to standard instructions. Further, we applied phenol–chloroform–isoamyl alcohol as described in Delgado-Calle J et al (2012) Epigenetics 2012; 7: 83–91. Pubmed ID: 23124911.
|
| Label |
Cy3 and Cy5
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Data processing |
BeadStudio software v3.2.
Unmethylated and methylated signal intensities and detection p values included in Series supplementary file
|
| |
|
| Submission date |
Dec 23, 2014 |
| Last update date |
Jun 03, 2015 |
| Contact name |
Steve Horvath |
| E-mail(s) |
shorvath@altoslabs.com
|
| Organization name |
University of California, Los Angeles
|
| Department |
Human Genetics
|
| Lab |
Horvath
|
| Street address |
695 Charles E. Young Drive South, Box 708822
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095-7088 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE64490 |
DNA methylation profiles of human bone samples |
| GSE64511 |
DNA methylation profiles of multiple human tissues |
|
