|
| Status |
Public on Nov 19, 2014 |
| Title |
Human Female low BMD [miRNA_D4] |
| Sample type |
RNA |
| |
|
| Source name |
Healthy female
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Peripheral blood monocytes
Sex: female
|
| Treatment protocol |
Monocyte isolation ：A monocyte negative isolation kit (Order No. 130-091-153, Miltenyi Biotec Inc., Auburn, CA, USA) was used to isolate circulating monocytes from 150mL whole blood .
|
| Growth protocol |
One hundred and fifty milliliters of peripheral blood were drawn for each selected subject.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from monocytes was extracted using a Qiagen RNeasy Mini Kit .
|
| Label |
biotin
|
| Label protocol |
miRNA microarray was performed by Capitalbio Cor. (Beijing, China). Briefly, 1 μg of total RNA from each sample was end-tailed with Poly(A) and ligated to the biotinylated signal molecule (FlashTag™ Biotin RNA Labeling Kit).(dT24) primer to produce biotinylated cRNA. The full-length cRNAs were fragmented to 20 to 200 bp and hybridized to Affymetrix GeneChip Human Exon 1.0 ST Array. 100 ng of total RNA was amplified and labeled using the Affymetrix Whole-Transcript (WT) Sense Target Labeling Protocol without rRNA reduction.
|
| |
|
| Hybridization protocol |
Hybridization (Affymetrix Genechip Hybridization Oven 640) was carried out on Affymetrix genechip® miRNA 2.0.
|
| Scan protocol |
The hybridized chips were washed (Affymetrix Fluidics Station 450), stained with phycoerythrin-streptavidin, and scanned using an Affymetrix GeneChip® Scanner 3000 7G.
|
| Data processing |
Scanned images were quantified by GeneChip® Command Console® Software. miRNA QC Tool software was used for data summarization, normalization, and quality control.
|
| |
|
| Submission date |
Nov 19, 2014 |
| Last update date |
Nov 21, 2014 |
| Contact name |
shishi min |
| E-mail(s) |
minshishi1991@qq.com
|
| Phone |
15580884581
|
| Organization name |
Hunan Normal University
|
| Department |
College of life sciences
|
| Lab |
Laboratory of Molecular and Statistical Genetics
|
| Street address |
Lu shan road
|
| City |
Chang sha |
| State/province |
Hunan |
| ZIP/Postal code |
410000 |
| Country |
China |
| |
|
| Platform ID |
GPL14613 |
| Series (2) |
| GSE62589 |
Integrative Analysis of Transcriptomic and Epigenomic Data to Reveal Regulation Patterns for Osteoporosis Risk |
| GSE63446 |
Integrative Analysis of Transcriptomic and Epigenomic Data to Reveal Regulation Patterns for Osteoporosis Risk [miRNA] |
|
