|
| Status |
Public on Mar 02, 2015 |
| Title |
genomic DNA from Brain MidBrain Sample32 |
| Sample type |
genomic |
| |
|
| Source name |
Brain MidBrain
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Brain MidBrain
age: 58
Sex: F
downsyndrome.status: N
dnamage: 39.91284293
ageaccelerationdsvscontrols: -6.18791183087534
ageaccelerationdsvscontrolsincrbm: NA
ageaccelerationdsvscontrolsinfrontalcortex: NA
ageaccelerationdsvscontrolsotherregions: -5.1378470461047
ageaccelerationresidual: -7.65986741718497
postmorteminterval: 16
|
| Treatment protocol |
Standard Illumina Protocol
|
| Growth protocol |
DNA extracted from fresh frozen brain tissue
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted and purified from fresh frozen tissue using AllPrep DNA/RNA mini kit according to standard instructions
|
| Label |
Cy3 and Cy5
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Data processing |
Unmethylated and methylated signal intensities and detection p values in datSignal.csv supplementary file
BeadStudio software v3.2.
|
| |
|
| Submission date |
Nov 17, 2014 |
| Last update date |
Mar 02, 2015 |
| Contact name |
Steve Horvath |
| E-mail(s) |
shorvath@altoslabs.com
|
| Organization name |
University of California, Los Angeles
|
| Department |
Human Genetics
|
| Lab |
Horvath
|
| Street address |
695 Charles E. Young Drive South, Box 708822
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095-7088 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE63347 |
DNA methylation profiles of brain samples from Down Syndrome subjects and controls. |
|
