|
Status |
Public on Mar 02, 2015 |
Title |
genomic DNA from Brain CRBM Sample18 |
Sample type |
genomic |
|
|
Source name |
Brain CRBM
|
Organism |
Homo sapiens |
Characteristics |
tissue: Brain CRBM age: 59 Sex: F downsyndrome.status: N dnamage: 48.64227487 ageaccelerationdsvscontrols: 1.82243425242332 ageaccelerationdsvscontrolsincrbm: 1.74282919485476 ageaccelerationdsvscontrolsinfrontalcortex: NA ageaccelerationdsvscontrolsotherregions: NA ageaccelerationresidual: 0.425006317952502 postmorteminterval: 10
|
Treatment protocol |
Standard Illumina Protocol
|
Growth protocol |
DNA extracted from fresh frozen brain tissue
|
Extracted molecule |
genomic DNA |
Extraction protocol |
genomic DNA was extracted and purified from fresh frozen tissue using AllPrep DNA/RNA mini kit according to standard instructions
|
Label |
Cy3 and Cy5
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
Data processing |
Unmethylated and methylated signal intensities and detection p values in datSignal.csv supplementary file BeadStudio software v3.2.
|
|
|
Submission date |
Nov 17, 2014 |
Last update date |
Mar 02, 2015 |
Contact name |
Steve Horvath |
E-mail(s) |
shorvath@altoslabs.com
|
Organization name |
University of California, Los Angeles
|
Department |
Human Genetics
|
Lab |
Horvath
|
Street address |
695 Charles E. Young Drive South, Box 708822
|
City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90095-7088 |
Country |
USA |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE63347 |
DNA methylation profiles of brain samples from Down Syndrome subjects and controls. |
|