|
| Status |
Public on Nov 06, 2015 |
| Title |
221_lesioned |
| Sample type |
genomic |
| |
|
| Source name |
Lesioned cartilage
|
| Organism |
Homo sapiens |
| Characteristics |
age: 80.6
joint: Knee
Sex: Female
tissue: Lesioned cartilage
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was isolated using Promega Wizard Genomic DNA Purification kits
|
| Label |
Cy3 and Cy5
|
| Label protocol |
standard Illumina protocol
|
| |
|
| Hybridization protocol |
Bisulfite-converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 BeadChips using the standard Illumina protocol.
|
| Scan protocol |
Arrays were imaged using High Scan SQ using the standard recommended Illumina scanner setting.
|
| Description |
DNA was BS treated using Zymo EZ-96 DNA Methylation kits
|
| Data processing |
Inhouse quality control pipeline, takes into account color bias and the different probe types.
|
| |
|
| Submission date |
Nov 07, 2014 |
| Last update date |
Nov 06, 2015 |
| Contact name |
Wouter den Hollander |
| E-mail(s) |
w.denhollander@lumc.nl
|
| Organization name |
LUMC
|
| Department |
Molecular Epidemiology
|
| Street address |
Einthovenweg 20
|
| City |
Leiden |
| ZIP/Postal code |
2333ZC |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE63106 |
Assessing transcriptionally functional CpGs in articular cartilage |
|
