|
Status |
Public on Nov 06, 2015 |
Title |
125_preserved |
Sample type |
genomic |
|
|
Source name |
Preserved cartilage
|
Organism |
Homo sapiens |
Characteristics |
age: 65.7 joint: Knee Sex: Female tissue: Preserved cartilage
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was isolated using Promega Wizard Genomic DNA Purification kits
|
Label |
Cy3 and Cy5
|
Label protocol |
standard Illumina protocol
|
|
|
Hybridization protocol |
Bisulfite-converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 BeadChips using the standard Illumina protocol.
|
Scan protocol |
Arrays were imaged using High Scan SQ using the standard recommended Illumina scanner setting.
|
Description |
DNA was BS treated using Zymo EZ-96 DNA Methylation kits
|
Data processing |
Inhouse quality control pipeline, takes into account color bias and the different probe types.
|
|
|
Submission date |
Nov 07, 2014 |
Last update date |
Nov 06, 2015 |
Contact name |
Wouter den Hollander |
E-mail(s) |
w.denhollander@lumc.nl
|
Organization name |
LUMC
|
Department |
Molecular Epidemiology
|
Street address |
Einthovenweg 20
|
City |
Leiden |
ZIP/Postal code |
2333ZC |
Country |
Netherlands |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE63106 |
Assessing transcriptionally functional CpGs in articular cartilage |
|