|
| Status |
Public on Sep 30, 2015 |
| Title |
N_insufficient_3_placenta |
| Sample type |
genomic |
| |
|
| Source name |
placenta (chorionic villi)
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
health state: Normal
maternal gestational weight gain (g): 6,200
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA extracted using QIAamp DNA Mini Kit (Qiagen)
|
| Label |
Cy3, Cy5
|
| Label protocol |
Standard Illumina labelling protocol
|
| |
|
| Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using the Illumina's iScan system using standard recommended scanner setting
|
| Data processing |
The intensity files (.idat), produced by the Illumina iScan system, were processed using Illumina GenomeStudio V2011.2 (Methylation Analysis Module v1.9.0) using Normalization to Internal Controls and Background Subtraction options. The average beta values were corrected using ComBat, an Empirical Bayes method-based statistical tool , to remove array-batch effects.
Unmethylated and methylated signal intensities
|
| |
|
| Submission date |
Oct 27, 2014 |
| Last update date |
Sep 30, 2015 |
| Contact name |
Kazuhiko Nakabayashi |
| E-mail(s) |
nakabaya-k@ncchd.go.jp
|
| Organization name |
National Research Institute for Child Health and Development
|
| Department |
Department of Maternal-Fetal Biology
|
| Street address |
2-10-1 Okura
|
| City |
Setagaya |
| State/province |
Tokyo |
| ZIP/Postal code |
157-8535 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE62733 |
Accumulation of epigenetic alterations at the promoters of transcriptional regulator genes in the placentas of pregnancy cases with inadequate maternal gestational weight gain |
|
