|
| Status |
Public on Oct 05, 2015 |
| Title |
t5.miR.14 |
| Sample type |
RNA |
| |
|
| Source name |
Hippocampal brain_MS5
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: male
age: postnatal day 9 (P9)
tissue: Hippocampal brain
treated with: 5 mg/kg morphine + stress (MS5)
|
| Biomaterial provider |
The University of Washington (Seattle, WA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed by the CHDD Genomics Core Laboratory followed by the manufacturer’s protocols using the GeneChip platform by Affymetrix.
|
| Label |
biotin
|
| Label protocol |
For microarray hybridization, RNA was processed for hybridization to Affymetrix microRNA 2.0 Arrays through a core facility in the CHDD Genomics Core Laboratory at the University of Washington according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
The standard hybridization protocol was used as recommended by Affymterix.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 through the University of Washington Functional Genomics Laboratory CHDD Genomics Core Laboratory.
|
| Description |
Hippocampal brain
|
| Data processing |
Bioconductor software, RMA normalization log2
|
| |
|
| Submission date |
Oct 15, 2014 |
| Last update date |
Sep 05, 2017 |
| Contact name |
James William MacDonald |
| E-mail(s) |
jmacdon@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Environmental and Occupational Health Sciences
|
| Street address |
4225 Roosevelt Way NE
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98105-6099 |
| Country |
USA |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE62346 |
Neonatal morphine exposure alters mRNA and microRNA (miR) expression in mouse hippocampus |
|
