|
| Status |
Public on Nov 06, 2014 |
| Title |
B160-ES |
| Sample type |
genomic |
| |
|
| Source name |
Embryonic stem cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Embryonic stem cell line
cell type: Undifferentiated embryonic stem cells
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated (High Pure PCR Template Preparation Kit, Roche) and bisulfite converted (EZDNA Methylation Kit, Zymo Research) according to the manufacturers' protocol
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina protocol
|
| Scan protocol |
HumanMethylation450 BeadChips were scanned with Illumina's iScan System
|
| Description |
8691803157_R03C02
|
| Data processing |
IDAT files were processed and normalized by subset-quantile within array normalization (SWAN) and adjusted for batch effects using the R package ChAMP (version 1.2.7)
|
| |
|
| Submission date |
Sep 16, 2014 |
| Last update date |
Nov 06, 2014 |
| Contact name |
Ido Sagi |
| Organization name |
The Hebrew University of Jerusalem
|
| Department |
Genetics
|
| Lab |
Nissim Benvenisty Lab, The Azrieli Center for Stem Cells and Genetic Research
|
| Street address |
Givat Ram
|
| City |
Jerusalem |
| ZIP/Postal code |
91904 |
| Country |
Israel |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE61461 |
Coding mutations and loss-of-imprinting in human pluripotent cells derived by nuclear transfer and defined factors [DNA methylation profiling] |
| GSE61657 |
Coding mutations and loss-of-imprinting in human pluripotent cells derived by nuclear transfer and defined factors |
|
