|
| Status |
Public on Nov 03, 2014 |
| Title |
genomic DNA from human adipose of subject S11a15972 |
| Sample type |
genomic |
| |
|
| Source name |
Adipose
|
| Organism |
Homo sapiens |
| Characteristics |
subjectid: S11a15972
Sex: female
dna.extraction.method: AllPrep
age: 42
dnamage: 53.73099941
ageaccelerationresidual: 3.49785324444826
bmi: 70.2
diseasestatus: NASH
nafldactivityscore: 5
liversteatosis: 3
liverinflammation: 2
fibrosis: 3
|
| Treatment protocol |
Standard Illumina Protocol
|
| Growth protocol |
DNA extracted from fresh frozen tissue
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
For homogenisation of five to ten milligram frozen tissue and subsequent nucleic acid isolation, tubes with 1.4 mm ceramic beads (Precellys, Villeurbanne, France) and the AllPrep DNA/RNA Mini Kit (QIAGEN, Hilden, Germany) were used.
|
| Label |
Cy3, Cy5
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
genomic DNA from human adipose tissue
|
| Data processing |
BeadStudio software v3.2.
|
| |
|
| Submission date |
Sep 09, 2014 |
| Last update date |
Nov 03, 2014 |
| Contact name |
Steve Horvath |
| E-mail(s) |
shorvath@altoslabs.com
|
| Organization name |
University of California, Los Angeles
|
| Department |
Human Genetics
|
| Lab |
Horvath
|
| Street address |
695 Charles E. Young Drive South, Box 708822
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095-7088 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE61256 |
Obesity accelerates epigenetic aging of human liver |
| GSE61257 |
Human adipose Illumina Inf 450 DNA methylation data |
|
