|
| Status |
Public on Nov 12, 2014 |
| Title |
genomic DNA peripheral blood DNA p2E12 |
| Sample type |
genomic |
| |
|
| Source name |
peripheral blood DNA
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
sample type: peripheral blood DNA
disease state: normal
duplicate: 0
plate: p2
plate_destination: E12
row: E
col: 12
ChIP: 16
label: p2E12
agegap: 0
agebloodtaken: 62
round: 2
subjectid for matching: 92
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA samples were extracted from buffy coats using DNA Blood Mini Kits (Qiagen, Crawley, UK) according to standard instructions
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation450k Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
normal blood sample
|
| Data processing |
BeadStudio software v3.2
The in vitro methylated control samples IVM100 and IVM0 could not be pre-processed with the actual samples, but they are represented in Matrix_raw_signal.txt (linked as supplementary file on Series record).
|
| |
|
| Submission date |
Sep 05, 2014 |
| Last update date |
Nov 12, 2014 |
| Contact name |
James M Flanagan |
| E-mail(s) |
j.flanagan@imperial.ac.uk
|
| Organization name |
Imperial College London
|
| Department |
Surgery and Cancer
|
| Lab |
Epigenetics Unit
|
| Street address |
Hammersmith Hospital, Du Cane Rd
|
| City |
London |
| State/province |
London |
| ZIP/Postal code |
W12 0NN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE61151 |
Temporal stability and determinants of white blood cell DNA methylation in the Breakthrough Generations Study |
|
