|
Status |
Public on Nov 12, 2014 |
Title |
genomic DNA peripheral blood DNA p2B1 |
Sample type |
genomic |
|
|
Source name |
peripheral blood DNA
|
Organism |
Homo sapiens |
Characteristics |
gender: Female sample type: peripheral blood DNA disease state: normal duplicate: 0 plate: p2 plate_destination: B1 row: B col: 1 ChIP: 9 label: p2B1 agegap: 6 agebloodtaken: 67 round: 2 subjectid for matching: 47
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA samples were extracted from buffy coats using DNA Blood Mini Kits (Qiagen, Crawley, UK) according to standard instructions
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation450k Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
Description |
normal blood sample
|
Data processing |
BeadStudio software v3.2 The in vitro methylated control samples IVM100 and IVM0 could not be pre-processed with the actual samples, but they are represented in Matrix_raw_signal.txt (linked as supplementary file on Series record).
|
|
|
Submission date |
Sep 05, 2014 |
Last update date |
Nov 12, 2014 |
Contact name |
James M Flanagan |
E-mail(s) |
j.flanagan@imperial.ac.uk
|
Organization name |
Imperial College London
|
Department |
Surgery and Cancer
|
Lab |
Epigenetics Unit
|
Street address |
Hammersmith Hospital, Du Cane Rd
|
City |
London |
State/province |
London |
ZIP/Postal code |
W12 0NN |
Country |
United Kingdom |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE61151 |
Temporal stability and determinants of white blood cell DNA methylation in the Breakthrough Generations Study |
|