|
| Status |
Public on Aug 28, 2014 |
| Title |
male ethanol socially-enriched amygdala replicate 2 [miRNA] |
| Sample type |
RNA |
| |
|
| Source name |
male ethanol socially-enriched amygdala
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Long Evans
gender: male
age: postnatal day 42
brain region: Amygdala
prenatal exposure: Ethanol
postnatal social treatment: Socially-enriched
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen total RNA
|
| Label |
biotin
|
| Label protocol |
250 ng of total RNA was prepared for hybridization using the using the FlashTag Biotin HSR RNA Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Affymetrix EukGE-WS2v4 Protocol
|
| Scan protocol |
Standard Affymetrix Genechip 7G Scanner
|
| Description |
mesa_62
|
| Data processing |
Partek Genomics Suite 6.5 to perform RMA normalization; the data were filtered afterwards to include only rat transcripts since the experiments were done with rat RNA.
|
| |
|
| Submission date |
Aug 27, 2014 |
| Last update date |
Aug 28, 2014 |
| Contact name |
Frank A Middleton |
| E-mail(s) |
middletf@upstate.edu
|
| Phone |
315-464-7721
|
| Organization name |
SUNY Upstate Medical Univ
|
| Department |
Neuroscience and Physiology
|
| Street address |
505 Irving Avenue
|
| City |
Syracuse |
| State/province |
NY |
| ZIP/Postal code |
13210 |
| Country |
USA |
| |
|
| Platform ID |
GPL14613 |
| Series (2) |
| GSE60819 |
Effects of acute prenatal exposure to ethanol on microRNA expression are ameliorated by environmental manipulation. |
| GSE60901 |
Comparison of small RNA-seq and microarray analysis for determining the effects of acute prenatal ethanol exposure on microRNA expression and its amelioration by environmental manipulation |
|
