We differeniated human pluripotent stem cell lines by using StemPro-34 (Invitrogen) based media for 15 days.Cytokines or a chemical compound were utilized as follows during differentiation: on day 0-1, human BMP4 (10 ng/ml) and Rock-inhibitor (Y27632) (10 μM); on day 1-4, human BMP4 (10 ng/ml) and human basic fibroblast growth factor (bFGF; 5 ng/ml); on day 4-8, human vascular endothelial growth factor (hVEGF; 10 ng/mL), hbFGF (1 ng/mL), human interleukin-6 (hIL-6; 10 ng/mL), hIL-3 (40 ng/mL), hIL-11 (5 ng/mL), human stem cell factor (hSCF; 100 ng/mL), and human Fms-like tyrosine kinase 3 ligand (hFlt3L; 100 ng/mL); and on Day 8-15, hVEGF (10 ng/mL), hIL-6 (10 ng/mL), hIL-3 (40 ng/mL), hIL-11 (5 ng/mL), hSCF (100 ng/mL), hFlt3L (100 ng/mL), human erythropoietin (hEPO; 4U/ml), and human thrombopoietin (50 ng/mL)
Extracted molecule
genomic DNA
Extraction protocol
GenomicDNA lysates were extracted with DNeasy(R) blood and tissue kit (QIAgen) in accordance with manufacture's protocol.
Label
Cy5 and Cy3
Label protocol
Standard Illumina Protocol
Hybridization protocol
Standard Illumina protocol
Scan protocol
Standard recommended Illumina scanner setting protocol using BeadArray Reader
Reprogramming-associated aberrant DNA methylation determines hematopoietic differentiation capacity of human induced pluripotent stem cells [PSCderived_HPCs_methylation]
