|
Status |
Public on Nov 29, 2014 |
Title |
F_BS_X280_BS |
Sample type |
genomic |
|
|
Source name |
final female sample from blood_spot
|
Organism |
Homo sapiens |
Characteristics |
healthy_vs_disease: healthy sentrix_id: 9274651175 sentrix_position: R05C02 xist_avgs_dnam: 0.661115072185472 sex_code: F tissue_id: BS tissue_name: blood_spot sample in final analysis: final female sample
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA from WhatmanTM 903 Protein Saver Cards was isolated using GenSolve DNA Recovery Kit (GVR100, IntegenX), cleaned with the QiAmp DNA Micro Kit (Qiagen). and concentrated using Microcon DNA Fast Flow Devices, MRCF0R100 (Millipore).
|
Label |
Cy3 and Cy5
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
Scan protocol |
BeadChips were imaged using HiScan with standard recommended Illumina scanner setting
|
Data processing |
Raw intensities extracted using GenomeStudio v2010.1. Background normalization was conducted by subtracting averaged negative probe intensity from signal A and B.
|
|
|
Submission date |
Aug 08, 2014 |
Last update date |
Nov 29, 2014 |
Contact name |
Carolyn J Brown |
E-mail(s) |
cbrown@ubc.ca
|
Organization name |
University of British Columbia
|
Department |
Medical Genetics
|
Lab |
Brown Lab
|
Street address |
2350 Health Sciences Mall
|
City |
Vancouver |
State/province |
British Columbia |
ZIP/Postal code |
V6T1Z3 |
Country |
Canada |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE60275 |
Landscape of DNA methylation on the X chromosome reflects CpG density, functional chromatin state and X-chromosome inactivation. |
|