|
| Status |
Public on Nov 29, 2014 |
| Title |
F_PM_KIGA044r |
| Sample type |
genomic |
| |
|
| Source name |
final female sample from PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
given_sex: F
ethnicity: Bakiga
healthy_vs_disease: healthy
sentrix_id: 6929742105
sentrix_position: R03C02
replicated_redone: redone sample
xist_avgs_dnam: 0.60498991400694
sex_code: F
tissue_id: PM
tissue_name: PBMC
sample in final analysis: final female sample
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated using Qiagen AllPrep DNA/RNA mini kit according to manufacturer's instructions.
|
| Label |
Cy3 and Cy5
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
BeadChips were imaged using HiScan with standard recommended Illumina scanner setting
|
| Data processing |
Raw intensities extracted using GenomeStudio v2010.1. Background normalization was conducted by subtracting averaged negative probe intensity from signal A and B.
|
| |
|
| Submission date |
Aug 08, 2014 |
| Last update date |
Nov 29, 2014 |
| Contact name |
Carolyn J Brown |
| E-mail(s) |
cbrown@ubc.ca
|
| Organization name |
University of British Columbia
|
| Department |
Medical Genetics
|
| Lab |
Brown Lab
|
| Street address |
2350 Health Sciences Mall
|
| City |
Vancouver |
| State/province |
British Columbia |
| ZIP/Postal code |
V6T1Z3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE60275 |
Landscape of DNA methylation on the X chromosome reflects CpG density, functional chromatin state and X-chromosome inactivation. |
|
