|
Status |
Public on Jan 06, 2015 |
Title |
genomic DNA from Surgical resection GBM-1437 |
Sample type |
genomic |
|
|
Source name |
Surgical resection GBM-1437
|
Organism |
Homo sapiens |
Characteristics |
gender: M disease state: GBM geo expression data: GSM187167 age: 48 os: 16 survival_status: 1 mgmt_status: U
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was isolated using Qiagen AllPrep DNA/RNA Kit (Qiagen, 80204).
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
Data processing |
BeadStudio software v3.2 was used for primary data acqusition. Raw data was imported into R and further processed using R/Bioconductor package “methylumi”. The raw pre-processing (Illumina method) was used for data normalization.
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|
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Submission date |
Aug 08, 2014 |
Last update date |
Jan 06, 2015 |
Contact name |
Sebastian Kurscheid |
E-mail(s) |
sebastian.kurscheid@anu.edu.au
|
Organization name |
Australian National University
|
Department |
JCSMR
|
Lab |
Prof David Tremethick
|
Street address |
57 Garran Road
|
City |
Acton |
State/province |
ACT |
ZIP/Postal code |
2601 |
Country |
Australia |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE60274 |
Genome-wide DNA methylation profiling of 68 GBM (primary & recurrent), 4 glioma spheres and 5 non-tumor brain samples |
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