|
| Status |
Public on Mar 12, 2015 |
| Title |
CB_WT_2 |
| Sample type |
RNA |
| |
|
| Source name |
cerebellum
|
| Organism |
Mus musculus |
| Characteristics |
age: 8-10 weeks
brain region: cerebellum
genotype: Mecp2 +/y
|
| Growth protocol |
Mice were housed under standard conditions in the animal facility
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cerebellum was dissected from 8-10 week old MeCP2 R306C or wild-type littermate controls. RNA was trizol and extracted using Qiagen Rneasy purification with on column DNase steps.
|
| Label |
biotin
|
| Label protocol |
Fragmentation and labeling were carried out using the The Ambion® WT Expression Kit
|
| |
|
| Hybridization protocol |
Hybridization was carried out as described in the GeneChip® Whole Transcript (WT) Sense Target Labeling Assay Manual v4 Chapter 5
|
| Scan protocol |
Arrays were scanned with the Affymetrix GeneChip Scanner 3000
|
| Data processing |
Data were processed using Genespring software (Agilent). Gene level expression were summarized use RMA16.
probe group file: MoEx-1_0-st-v1.r2.pgf
meta-probeset file: MoEx-1_0-st-v1.r2.dt1.mm9.core.mps
|
| |
|
| Submission date |
Aug 04, 2014 |
| Last update date |
Mar 12, 2015 |
| Contact name |
Harrison Wren Gabel |
| E-mail(s) |
gabelh@pcg.wustl.edu
|
| Organization name |
Harvard Medical School
|
| Department |
Neurbiology
|
| Lab |
Michael Greenberg
|
| Street address |
220 longwood avenue
|
| City |
brookline |
| State/province |
Massachusetts |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL6096 |
| Series (2) |
| GSE60074 |
Length-dependent gene misregulation in Rett syndrome (MeCP2) |
| GSE60077 |
Length-dependent gene misregulation in Rett syndrome |
|
