|
| Status |
Public on May 09, 2015 |
| Title |
aldesterone-producing adenoma (APA) tissue from Case3 [methylation] |
| Sample type |
genomic |
| |
|
| Source name |
aldesterone-producing adenoma (APA)
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
subject: patients with aldosterone-producing adenoma (APA)
health state: primary aldosteronism
tissue type: aldesterone-producing adenoma (APA)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated using All Prep DNA/RNA Mini kit (Qiagen, Valencia, CA) according to the manufacturer’s instruction.
|
| Label |
Cy3, Cy5
|
| Label protocol |
Standard Illumina labelling protocol
|
| |
|
| Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using High Scan SQ using standard recommended Illumina scanner setting
|
| Description |
Case3_APA
|
| Data processing |
Genome Studio V2011.2; the non_normalized.txt contains unmethylated and methylated signal intensities.
Detection P-value and normalized average beta value. The methylation level of each of the > 480,000 CpG sites was calculated using the GenomeStudio Methylation Module 1.9.0 (Illumina). Normalization was performed using Normalization to Internal Controls and Background Subtraction options implemented in the Methylation Module 1.9.0.
|
| |
|
| Submission date |
Aug 04, 2014 |
| Last update date |
May 09, 2015 |
| Contact name |
Kazuhiko Nakabayashi |
| E-mail(s) |
nakabaya-k@ncchd.go.jp
|
| Organization name |
National Research Institute for Child Health and Development
|
| Department |
Department of Maternal-Fetal Biology
|
| Street address |
2-10-1 Okura
|
| City |
Setagaya |
| State/province |
Tokyo |
| ZIP/Postal code |
157-8535 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE60043 |
Integration of transcriptome and methylome analyses to dissect molecular pathogenesis of primary aldosteronism: comparision between aldosterone-producing adenomas and adjacent adrenal glands [methylome] |
| GSE60044 |
Integration of transcriptome and methylome analyses to dissect molecular pathogenesis of primary aldosteronism: comparision between aldosterone-producing adenomas and adjacent adrenal glands |
|
