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Status |
Public on May 09, 2015 |
Title |
aldesterone-producing adenoma (APA) tissue from Case1 [methylation] |
Sample type |
genomic |
|
|
Source name |
aldesterone-producing adenoma (APA)
|
Organism |
Homo sapiens |
Characteristics |
gender: female subject: patients with aldosterone-producing adenoma (APA) health state: primary aldosteronism tissue type: aldesterone-producing adenoma (APA)
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was isolated using All Prep DNA/RNA Mini kit (Qiagen, Valencia, CA) according to the manufacturer’s instruction.
|
Label |
Cy3, Cy5
|
Label protocol |
Standard Illumina labelling protocol
|
|
|
Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using High Scan SQ using standard recommended Illumina scanner setting
|
Description |
Case1_APA
|
Data processing |
Genome Studio V2011.2; the non_normalized.txt contains unmethylated and methylated signal intensities. Detection P-value and normalized average beta value. The methylation level of each of the > 480,000 CpG sites was calculated using the GenomeStudio Methylation Module 1.9.0 (Illumina). Normalization was performed using Normalization to Internal Controls and Background Subtraction options implemented in the Methylation Module 1.9.0.
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Submission date |
Aug 04, 2014 |
Last update date |
May 09, 2015 |
Contact name |
Kazuhiko Nakabayashi |
E-mail(s) |
nakabaya-k@ncchd.go.jp
|
Organization name |
National Research Institute for Child Health and Development
|
Department |
Department of Maternal-Fetal Biology
|
Street address |
2-10-1 Okura
|
City |
Setagaya |
State/province |
Tokyo |
ZIP/Postal code |
157-8535 |
Country |
Japan |
|
|
Platform ID |
GPL13534 |
Series (2) |
GSE60043 |
Integration of transcriptome and methylome analyses to dissect molecular pathogenesis of primary aldosteronism: comparision between aldosterone-producing adenomas and adjacent adrenal glands [methylome] |
GSE60044 |
Integration of transcriptome and methylome analyses to dissect molecular pathogenesis of primary aldosteronism: comparision between aldosterone-producing adenomas and adjacent adrenal glands |
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