|
| Status |
Public on Jan 01, 2015 |
| Title |
9020331094_R04C01 |
| Sample type |
genomic |
| |
|
| Source name |
gDNA from normal PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
gender: MALE
phenotype: Peanut.allergic
challenge outcome: allergic
|
| Treatment protocol |
NA
|
| Growth protocol |
untouched cells rested for 48 hours in RPMI media (serum free)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA purified using Qiagen All prep column extraction method
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Cy5 and Cy3
|
| |
|
| Hybridization protocol |
Infinium Methylation Assay according to manufacturers protocol (Illumina)
|
| Scan protocol |
iScan system
|
| Data processing |
idat files processed in Minfi normalized using the stratified quantile normalization method. Sex probes removed, cross hybridizing probes removed, poor quality probes removed, SNP probes (MAF < 0.05 at extension site) removed
|
| |
|
| Submission date |
Aug 01, 2014 |
| Last update date |
Jan 01, 2015 |
| Contact name |
David Martino |
| E-mail(s) |
david.martino@telethonkids.org.au
|
| Phone |
0863191635
|
| Organization name |
Telethon Kids Institute
|
| Street address |
Perth Childrens Hospital, 15 Hospital Avenue
|
| City |
NEDLANDS |
| State/province |
Western Australia |
| ZIP/Postal code |
6009 |
| Country |
Australia |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE59999 |
Blood DNA methylation biomarkers predict clinical reactivity in food-sensitized infants |
|
