|
| Status |
Public on Sep 30, 2014 |
| Title |
Genomic DNA from monocyte_3 |
| Sample type |
genomic |
| |
|
| Source name |
monocye
|
| Organism |
Homo sapiens |
| Characteristics |
ID: 89500
cell type: monocye
|
| Growth protocol |
Monocytes were cultured in the presence of GM-CSF (1000U/ml) and IL-4 (500U/ml) for 4 days to induce differentiation into mostly immature monocyte-derived DCs. These DCs were matured by culturing for an additional two days in the presence of Jonuleit cocktail (IL1β, IL1α, IL6, TNFα and PGE2)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using Qiagen Allprep micro kit according to manufacturer’s instructions.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium HumanMethylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
Sample 3
monocyte isolated from human blood
|
| Data processing |
BeadStudio software v1.2
GSGX Version: 1.1.0
Report Date: 7/25/14 13:03
Project: Hong450_illuminaNormBgrd
Group Set: new
Analysis: hong450_illuminaNormBgrd
Normalization: controls
|
| |
|
| Submission date |
Jul 27, 2014 |
| Last update date |
Sep 30, 2014 |
| Contact name |
Hong Ji |
| E-mail(s) |
hgji@ucdavis.edu
|
| Organization name |
University of California Davis
|
| Street address |
County Rd 98 & Hutchison Drive
|
| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE59796 |
DNA methylation dynamics during ex vivo differentiation and maturation of human dendritic cells |
|
